The overall challenge of Thrust 3 is to improve understanding of the effect of physical and chemical cues of surfaces on cellular behavior across a range of length scales. The knowledge gained will be used to develop in-silico models that will enable accelerated design and fabrication of new interactive biomaterials and devices. Biomaterials are synthetic or natural substances that are engineered to interact directly with biological systems and influence structural functions. Recently, major advances in cellular and molecular technology have resulted in a new generation of smart biomaterials. While cells can respond to chemical features of underlying substrates containing domains as small as sub-nm, it is the topographical features of the substrates, from a nanometer to mesoscale, that effectively modulate many responses including adhesion, migration, proliferation, differentiation, metabolism and apoptosis.

The Thrust 3 challenges will be addressed systematically. During the first two years, new building blocks will be prepared and surface modified where appropriate. As these are made available, we will begin assembling them into 3D structures. In all cases, key chemical, rheological, and topological data will be collected and sent to the MCC for inclusion into databases. Beginning with simple, known materials, we will be developing protocols for measuring cellular response to surfaces and fabricated structures. As new materials become available, these will be tested similarly and the results sent back to the MCC for expanding databases and for the development of design tools. As the program progresses, tools from the MCC will enable the design and construction of second generation materials and fabricated structures. These, in turn, will undergo chemical and biological testing. Results from simulations will inform the design of second generation materials in advanced years of the project.

JOURNAL PUBLICATIONS

Year 1

• St John, F.J.; Dietrich, D.; Crooks, C.; Balogun. P.; de Serrano, V.; Pozharski, E.; Smith J.K.; Bales, E.; Hurlbert, J.(2018). A plasmid borne, functionally novel glycoside hydrolase family 30 subfamily 8 endoxylanase from solventogenic Clostridium. Biochem J. 475 (9) 1533 to 1551
• Pan, Panpan; Chen, Xiao; Metavarayuth, Kamolrat; Su, Jiacan; Wang, Qian(2018). Self-assembled supramolecular systems for bone engineering applications. Current Opinion in Colloid Interface Science. 35, 104-111
• Pageni, Parasmani; Yang, Peng; Chen, Yung Pin; Huang, Yucheng; Bam, Marpe; Zhu, Tianyu; Nagarkatti, Mitzi; Benicewicz, Brian C.; Decho, Alan W.; and Tang, Chuanbing(2018). Charged Metallopolymer-Grafted Silica Nanoparticles for Antimicrobial Applications. Biomacromolecules. 19(2), 417-425
• Lamm, Meghan E.; Wang, Zhongkai; Zhou, Jiangjun; Yuan, Liang; Zhang, Xinzhou; Tang, Chuanbing (2018). Sustainable epoxy resins derived from plant oils with thermo- and chemo-responsive shape memory behavior. Polymer. 144(), 121-127
• Zhang, Libo; Xu, Yanmei; Makris, Thomas; Wang, Qian(2018). Enhanced Arylamine NOxygenase Activity of PolymerEnzyme Assemblies by Facilitating Electron-Transferring Efficiency. Biomacromolecules, 19, 3, 918-925

Year 2

• Kuang, Serena Y.; Wang, Zhonghai; Yang, Xiaoqi; Huang, Ting; Xi, Tingfei; Gao, Bruce Z.(2018). Developmental Electrophysiology of Cultured Neuronal Networks at Early Stages. Biosensors and Bioelectronics Open Access, BBOA-122
• Na Bhuket, Ratnatilaka; Luckanagul, Jittima; Rojsitthisak, Pornchai; Wang, Qian(2018). Chemical Modification of Enveloped Viruses for Biomedical Applications.  Biol., 2018,10, 666-679
• Guo, Jiawang; Zhao, Xia; Hu, Jun; Lin, Yuan; Wang, Qian(2018). Tobacco mosaic virus with peroxidase-like activity for cancer cells detection through colorimetric assay.  Pharmaceutics, 2018, 15(8), 2946-2953
• Zhang, C.; Adler, P.H.; Monaenkova, D.; Andrukh, T.; Pometto, S., Beard, C.E., Kornev, K.G.(2018). Self-assembly of the butterfly proboscis: the role of capillary forces.  R. Soc. Interface 15: 20180229
• Liu, Tao; Zhao, Xia; Wang, Lei; Yang, Jia; Metavarayuth, Kamolrat; Lin, Yuan; Yuan, Jishan; Wang, Qian(2019). Using Plant Virus based Nanorods to Modulate the Differentiation of Human Bone Marrow Stem Cells. Journal of Biomedical Nanotechnology. 15(2), 363-372
• Coyle, Robert; Yao, Jenny; Richards, Dylan; Mei, Ying(2019). The Effects of Metabolic Substrate Availability on Human Adipose-Derived Stem Cell Spheroid Survival. Tissue Engineering Part A. Volume: 25 Issue 7-8: April 16, 2019. Online ahead of print.
• Tobias, Andrew; Rooke, William; Hanks, Timothy W.(2019). Incorporation of gold nanoparticles into the bilayer of polydiacetylene unilamellar vesicles. Colloid Polym Sci (2019) 297:85
• Aprelev, Pavel; Bruce, Terri; Beard, Charles; Adler, Peter; Kornev, Konstantin(2019). Nucleation and Formation of a Primary Clot in Insect Blood. Scientific Reports, 9:3451

Year 3

• Gan, Peiheng; Patterson, Michaela; Velasquez, Alexa; Wang, Kristy; Tian, Di; Windle, Jolene; Tao, Ge; Judge, Daniel; Makita, Takako; Park, Thomas; Sucov, Henry (2019). Tnni3k Alleles Influence Ventricular Mononuclear Diploid Cardiomyocyte Frequency. PLOS Genetics, 15(10), 1-24.
• He, Chen; Goodwin, Peter; Yunus, Ahmed; Dickson, Robert; Petty, Jeffrey(2019). A Split DNA Scaffold for a Green Fluorescent Silver Cluster. The Journal of Physical Chemistry, 123(28), 17588-17597.
• Kamolrat Metavarayuth, Panita Maturavongsadit, Xiao Chen, Pongkwan Sitasuwan, Lin Lu, Jiacan Su, and Qian Wang(2019). Nanotopographical Cues Mediate Osteogenesis of Stem Cells on Virus Substrates through BMP‑2 Intermediate. Nano Letters, 19(12), 8372-8380.
• Lu, Lin; Zhang, Libo; Yuan, Liang; Zhu, Tianyu; Chen, Wilfred; Wang, Guiren; Wang, Qian(2019). Artificial Cellulosome Complex From the Self-Assembly of Ni-NTA-Functionalized Polymeric Micelles and Cellulases. ChemBioChem, 20, 1394-1399.
• Rahman, Md Anisur; Cha, Yujin; Yuan, Liang; Pageni, Parasmani; Zhu, Tianyu; Jui, Moumita Sharmin; Tang, Chuanbing(2019). Polymerization‐induced Self‐assembly of Metallo‐polyelectrolyte Block Copolymers. Journal of Polymer Science, 58(1), 77-83.
• Rahman, Md Anisur; Jui, Moumita Sharmin; Bam, Marpe; Cha, Yujin; Luat, Edgar; Alabresm, Amjed; Nagarkatti, Mitzi; Decho, Alan W.; Tang, Chuanbing(2020). Facial Amphiphilicity-Induced Polymer Nanostructures for Antimicrobial Applications. ACS Applied Materials and Interfaces, 1-10.
• Sha, Ye; Zhang, Yudi; Xu, Enhua; McAlister, C. Wayne; Zhu, Tianyu; Craig, Stephen L.; Tang, Chuanbing(2019). Generalizing Metallocene Mechanochemistry to Ruthenocene Mechanophores. Chemical Science, 10(19), 1-7.
• Sha, Ye; Zhu, Tianyu; Rahman, Md Anisur; Cha, Yujin; Hwang, Jihyeon; Luo, Zhenyang; Tang, Chuanbing(2020). Synthesis of Site-specific Charged Metallopolymers via Reversible Addition-fragmentation Chain Transfer (RAFT) Polymerization. Polymer, 187, 1-8.
• Shen, Hua; Gan, Peiheng; Wang, Kristy; Darehzereshki, Ali; Wang, Kai; Kuma, S Ram; Lien, Ching-Ling; Patterson, Michaela; Tao, Ge; Sucov, Henry (2020). Mononuclear Diploid Cardiomyocytes Support Neonatal Mouse Heart Regeneration in Response to Paracrine IGF2 Signaling. eLife, 9, 1-24.
• Wright, Cassandra J.; Molino, Binbin Zhang; Chung; Johnson H.; Pannell, Jonathan T.; Kuester, Melissa; Molino, Paul J.; Hanks, Timothy W.(2020). Synthesis and 3D Printing of Conducting Alginate-Polypyrrole Ionomers. Gels, 6(2), 1-12.
• Wu, Jiang; Chen, Anqi; Zhou, Yajiao; Zheng, Sen; Yang, Yao; An, Ying; Xu, Ke; He, Huacheng; Kang, Jianming; Luckanagul, Jittima Amie; Xian, Ming; Xiao, Jian; Wang, Qian. (2019). Novel H2S-Releasing Hydrogel for Wound Repair via in situ Polarization of M2 Macrophages. Biomaterials, 222, 1-12.
• Yuan, Cai; Wang, Zhonghai; Borg, Thomas; Ye, Tong; Baicu, Catalin; Bradshaw, Amy; Zile, Michael; Runyan, Raymond; Shao, Yonghong; Gao, Bruce (2019). Changes in the Crystallographic Structures of Cardiac Myosin Filaments Detected by Polarization-dependent Second Harmonic Generation Microscopy. Biomedical Optics Express, 10(7), 3183-3195.
• Yuan, Cai; Zhao, Xiaolei; Wang, Zhonghai; Borg, Thomas; Ye, Tong; Khalpey, Zain; Runyan, Raymond; Shao, Yonghong; Gao, Bruce(2020). Study of the Expression Transition of Cardiac Myosin Using Polarization-Dependent SHG Microscopy. Biophysical Journal, 118, 1058-1066.
• Zhang, Chengqi; Adler, Peter H.; Kornev, Konstantin G.(2019). Insect Solutions for Open Self-Cleaning Microfluidics. Advanced Materials Interfaces, 6(23), 1-6.
• Zhang, Libo; Beatty, Andrew; Lu, Lin; Abdalrahman, Akrm; Makris, Thomas M; Wang, Guiren; Wang, Qian(2020). Microfluidic-assisted Polymer-protein Assembly to Fabricate Homogeneous Functionalnanoparticles. Materials Science and Engineering C, 111, 1-8.

CONFERENCE PAPERS, POSTERS OR PRESENTATIONS

Year 1

• Hurlbert, Jason, Presentation, 2017, Enzymatic Degradation of Hemicellulosic Polysaccharides for Industrial Applications, Materials Science and Technology, Pittsburgh, PA
• Mei, Ying, Presentation, 2018, Development of alginate-based vasculogenic hydrogel for biofabrication, MADE in SC Industrial Engagement and Advisory Board Meeting, Columbia, SC
• Schiller, Ulf, Presentation, 2018, Simulation-driven optimization of stents for treatment of brain aneurysms, International Conference on Discrete Simulation of Fluid Dynamics, Worcester, MA
• Wang, Fang; Schiller, Ulf, Presentation, 2018, Mesoscopic simulation of transport phenomena in fibrous porous media, International Conference for Mesoscopic Methods in Engineering in Science, Newark, DE

Year 2

• Radcliff, Jay, Poster, 2018, Synthesis and characterization of alginate-polypyrrole aerogels functionalized with dodecanethiol, Southeastern Regional Meeting of the American Chemical Society, Augusta, GA
• Drake, Andrew; Hanks, Timothy, Poster, 2018, Synthesis and characterization of alginate-polypyrrole aerogels functionalized with dodecanethiol, Southeastern Regional Meeting of the American Chemical Society, Augusta, GA
• Gregory, Arica; Miller, Emily; Alexander-Bryant, Angela, Poster, 2018, Implantable peptide-hydrogel drug delivery system for treating glioblastoma multiforme, Biomedical Engineering Society, Atlanta, GA
• Gregory, Arica; Miller, Emily; Alexander-Bryant, Angela, Presentation, 2018, Implantable peptide-hydrogel drug delivery system for treating glioblastoma multiforme, Biomedical Engineering Society, Atlanta, GA
• Miller, Emily; Alexander-Bryant, Angela, Poster, 2018, Liposomal Combination Drug and siRNA Delivery to Combat Drug-Resistant Ovarian Cancer, Biomedical Engineering Society, Atlanta, GA
• Miller, Emily; Alexander-Bryant, Angela, Presentation, 2018, Liposomal Combination Drug and siRNA Delivery to Combat Drug-Resistant Ovarian Cancer, Biomedical Engineering Society, Atlanta, GA
• Samac, Timothy; Alexander-Bryant, Angela, Poster, 2018, Comparison of Real-Time Imaging and Endpoint Analysis of Drug Therapeutic Efficacy in Glioblastoma Multiforme Cells, Biomedical Engineering Society, Atlanta, GA
• Samac, Timothy; Mbiki, Sarah; Gilmore, Jordon; Alexander-Bryant, Angela, Presentation, 2018, Comparison of Real-Time Imaging and Endpoint Analysis of Drug Therapeutic Efficacy in Glioblastoma Multiforme Cells, Biomedical Engineering Society, Atlanta, GA
• Yunus, Ahmed; Petty, Jeff, Poster, 2018, Fluorescence Anisotropy Studies of DNA-Fluorescence anisotropy studies of DNA-bound silver Clusters, Southeastern Regional Meeting of the American Chemical Society, Augusta, GA
• Brady, Cheyenne; Evers, Austin; Sahoo, Pabitra; Twiss, Jeffrey; Larsen, Jessica, Presentation, 2018, Optimization of Polymersomes for Delivery of Nerve-Regenerating Peptides, Biomedical Engineering Society, Atlanta, GA
• Hanks, Timothy, Presentation (invited), 2018, Functionalization of intrinsically conductive polymer films and nanoparticles by thiol-terminated hydrophilic polymers, Southeastern Regional Meeting of the American Chemical Society, Augusta, GA
• Larsen, Jessica, Presentation, 2018, Polymersomes Deliver Active Enzyme to the Brain of Felines as a Treatment of GM1 Neurodegeneration, Biomedical Engineering Society, Atlanta, GA
• Mei, Ying, Presentation, 2019, The development of vasculogenic hydrogel for bioprinting, Charleston Regenerative Medicine Workshop, Charleston, SC
• Ortitz Piccard, Selina; Chavis, Byrson; Cai, Li; Jiang, Hong; Wang, Qian, Presentation, 2018, Development of novel glycan-functionalized viral nanoparticles for cell targeting, Southeastern Regional Meeting of the American Chemical Society, Augusta, GA
• Wetherill, Richard; Hanks, Timothy, Presentation, 2018, Biologically-triggered release of organic dopants from functionalized intrinsically conducting polymer composites, Southeastern Regional Meeting of the American Chemical Society, Augusta, GA

Year 3

• Brown, Chanty; Gardner, Samanatha; Singleton, Elizabeth; Jessica, Jager; Marosok, Dominic; Ramirez, Yvette; Hamas, Taylor; Spitz, Jessica; Larsen, Jessica and Alexander-Bryant, Angela. Polymersomes facilitate transport of gold nanoparticles into neuroblastoma cells. Annual Biomedical Research Conference for Minority Scientists, Anaheim, SC, 2019.
• Elpers, Margaret; Gregory, Arica; Miller, Emily; Alexander-Bryant, Angela and Nukovic, Alexandra. Implantable peptide-hydrogel drug delivery system for treating glioblastoma multiforme. Biomedical Engineering Society, Philadelphia, PA, 2019.
• Fuller, Makala and Hanks, Tim. Control of encapsulation and embed magnetic nanoparticles within the aqueous interior bilayer of liposomes prepared from 10,12-pentacosadiynoic acid. ACS, Augusta, GA, 2019.
• Gao, Bruce and Adams, Meridith. Design of a Live 3D Bioprinter Monitoring System for Collagen Testing. Biomedical Engineering Society, Philadelphia, PA, 2019.
• Gao, Bruce and Ailin Wei. In VivoLike Intercalated Discs Achieved In A Neonatal Cardiomyocyte Culture Model. Biomedical Engineering Society, Philadelphia, PA, 2019.
• Gao, Bruce and Ailin Wei. Study of the sarcomeric addition process in a tissue-like cell construct under mechanical overload via TPEF-SHG imaging system. Photonics West-BiOS, San Francisco, CA, 2020.
• Gao, Bruce and Ailin Wei. Study of the sarcomeric addition process in a tissue-like cell construct under mechanical overload via TPEF-SHG imaging system. Photonics West-BiOS, San Francisco, CA, 2020.
• Gao, Bruce and Shenghao, Tan. Simulation of History Force on Microparticles under Optical Tweezers. Biomedical Engineering Society, Philadelphia, PA, 2019.
• Hanks, Tim. Anti-biofouling intrinsically conducting polymer films and nanoparticles. National Chemistry Laboratory, Pune, India, 0. (Invited Presentation)
• Hanks, Tim. Anti-biofouling surfaces from hydrophilic polymer brushes on intrinsically conducting polymer films and nanoparticles. Federation of African Societies of Chemistry, Gaborone, Botswana, 2019. (Invited Presentation)
• Hanks, Tim. Polydiacetylene nanostructures as sensor platforms. ACS, Augusta, GA, 2019. (Invited Presentation)
• Hanks, Tim; Feinberg, Hannah. Surface modification of titanium surfaces for improved tendon adhesion. ACS, Augusta, GA, 2019.
• Knepper, Allen and Hanks, Tim. Exploration of controlled release properties of polypyrrole films. ACS, Augusta, GA, 2019.
• Kornev, Konstantin. Magnetic rotational spectroscopy for analysis of insect blood.. Magnetism Life, Riga, Latvia, 2019. (Invited Presentation)
• Kornev, Konstantin; Aprelev, Pavel; Brasovs, Artis; Beard, Charles and Adler, Peter. Probing viscosity of insect blood at different spatial and time scales. Society of Integrative Comparative Biology Annual Meeting, Austin, TX, 2020.
• Mei, Ying and Barrs, Ryan. Development of vascular inductive bioinks with defined chemistry for vascularized tissue unit fabrication. Biomedical Engineering Society, Philadelphia, PA, 2019.
• Mei, Ying. The discovery of a novel laminin-derived RGD peptide for neovascularization. Biomedical Engineering Society, Philadelphia, PA, 2019.
• Mei, Ying; Silver, Sophia ; Barrs, Ryan and Yost, Michael. Biomaterials Development through Active Machine Learning Assessment of Vascular Morphogenesis. 2020 Charleston Regenerative Medicine Workshop, Charleston, SC, 2020.
• Nukovic, Alexandra; Gregory, Arica and Alexander-Bryant, Angela. Evaluation of a self-assembling peptide hydrogel for drug delivery into T98G glioblastoma cells. Biomedical Engineering Society, Philadelphia, PA, 2019.
• Outten, Franklin; Grossoehme, Nicholas and Satyam Singh. Characterization of a pH-responsive nanocage based on the ferritin iron storage protein. Southeastern Regional Meeting of the ACS, Savannah, GA, 2019.
• Outten, Franklin; Grossoehme, NIcholas; Brandon Ellison and Alexandra Perez. Measuring heat related to the dissociation and reformation of ferritin using isothermal titration calorimetry.. Southeastern Regional Meeting of the ACS, Savannah,, GA, 2019.
• Outten, Franklin; Grossoehme, NIcholas; Brandon Ellison and Alexandra Perez. Measuring heat related to the dissociation and reformation of ferritin using isothermal titration calorimetry.. Annual Meeting of SC INBRE, Columbia, SC, 2020.
• Owen, Tripp and Hanks, Tim. Modification of polysiloxane surface coatings with zwitterionic thiols using thiol-ene click. ACS, Augusta, GA, 2019.
• Parker, Blake; Hanks, Tim and Pennington, William. Reversible thermochromic alkali metal polydiacetylene microcrystals. ACS, Augusta, GA, 2019.
• Samec, Timothy; Boulos, Jessica and Alexander-Bryant, Angela. Design of a fusogenic peptide for delivery of bioactive siRNAs to ovarian cancer cells in vitro. Biomedical Engineering Society, Philadelphia, PA, 2019.
• Singleton, Elizabeth; Gardner, Samantha; Brown, Chanty; Jager, Jessica; Marosok, Dominic; Ramirez, Yvette; Hamas, Taylor; Spitz, Jessica; Larsen, Jessica and Alexander-Bryant, Angela. Polymersomes facilitate transport of gold nanoparticles into neuroblastoma cells. Biomedical Engineering Society, Philadelphia, PA, 2019.
• Stueber, Chris and Hanks, Tim. Modification of head-groups on 10,12-pentacosadiynoic acid using click chemistry. ACS, Augusta, GA, 2019
• Wang, Qian and Ayse Turhan Comert. Comparison of Differences in Cell Work Studies on Titanium-Based Substrates. , Columbia, SC, 2019.
• Wang, Zibo and Hanks, Tim. Surface modified polydopamine-polypyrrole coatings. ACS, Augusta, GA, 2019.